Realtime PCR
      Real-time fluorescence quantitative PCR technology is to add a fluorophore to the PCR reaction system, use the fluorescence signal accumulation to monitor the entire PCR process in real time, and finally pass the standard curve
Method for quantitative analysis of unknown templates. This technology not only achieves the quantification of DNA templates, but also has high sensitivity, greater specificity and reliability, and can achieve multiple
Heavy response, high degree of automation, no pollution, real-time and accuracy.
  Experimental steps:
     Sample acquisition, processing and preparation
 1.1 Suspension cells: Pour the suspension cells at 1 × 106 cells / mL into the centrifuge tube and centrifuge to pellet the cells. Rinse twice with PBS, centrifuge the bottom of the cells
 Add 500-1000 μl of Trizol and store in -80 degrees refrigerator. Or store the stem cells at the bottom of the centrifuge in a -80 degree refrigerator.
1.2 Adherent cells: Digest the cells 1 × 106 cells / mL with trypsin, rinse the cells with centrifugal PBS twice, centrifuge the bottom of the cells, and add 500-1000 μl Trizol to the centrifuge tube.
Store in -80 ° C refrigerator. Or store the stem cells at the bottom of the centrifuge in a -80 degree refrigerator.
1.3 Tissue: Collect 100mg of fresh tissue, put it into a centrifuge tube, mark it, freeze it for a while in liquid nitrogen, and store it in a -80 degree refrigerator.
1.4 Blood: 1mL of whole blood collected with anticoagulation tube, add RNA protection agent, make a mark, and store in -80 degree refrigerator.
    2. RNA or DNA quality control
    3. Reverse transcription
    4. Primer verification
    5. qPCR process
    6. Data analysis
  we provide:
  1. Extracted RNA and electropherograms and reverse transcribed cDNA samples
  2. Operating steps and detailed reagents and consumables
  Primer sequence
  4. Dissolution and amplification curves
  5. Raw data and formal experimental data after processing

  experiment equipment:


 
 
  Results diagram:


 

 
 
 
  Reminder:
      1. Cell samples above 106, stored at -80 degrees after collection; tissue samples 100mg samples; serum or blood above 1mL, transported on dry ice
      2. More than 10 samples of a single gene
      3. Test cycle does not include probe and standard synthesis time
      4. The company's default extraction method TRIZOL, if the kit extraction is required, the customer needs to provide