Introduction: TEM / SEM
Provider: Shanghai Baiyou Biological
Service Name: Transmission / Scanning Electron Microscope (TEM / SEM)
Region: Shanghai
 
Transmission electron microscope (TEM) is widely used in materials science and biology. Because the electrons are easily scattered or absorbed by the object, the penetrating power is low, and the density and thickness of the sample will affect the final imaging quality. Thinner ultra-thin sections must be prepared, usually 50-100nm. Therefore, the sample used for observation with a transmission electron microscope needs to be processed very thinly. Commonly used methods: ultra-thin sectioning method, frozen ultra-thin sectioning method, freezing etching method, freezing fracture method, etc. For liquid samples, observation is usually performed on a pre-treated copper wire. Optical microscope
    Scanning electron microscope (SEM) is a microscopic morphology observation method between transmission electron microscope and optical microscope, which can directly use the material properties of the surface material of the sample for microscopic imaging. Its advantages include: 1. It has a high magnification, continuously adjustable between 200,000 and 200,000 times; 2.It has a large depth of field, a large field of view, and a rich three-dimensional imaging. It can directly observe the uneven surface of various samples. Fine structure; 3, sample preparation is simple. The current scanning electron microscopes are equipped with X-ray spectrometer devices, which can simultaneously observe the microstructure and analyze the composition of micro-regions, so it is a very useful scientific research instrument today.
Service content Price Result content Remarks
TEM transmission electron microscope 800 yuan / sample 5 pictures / sample included Ultra-thin sections and sample processing. Special organization positioning
SEM scanning electron microscope 400 yuan / sample
Special positioning 80 yuan / time Provide pictures
Note: For electron microscopy testing services, customers need to provide glutaraldehyde-fixed samples. Negotiable based on sample size
SEM specimen requirements
1) The transmission electron microscope requires the customer to provide a tissue block of about 1mm3, and the material acquisition site must be as accurate as possible.
2) The scanning electron microscope requires the customer to provide a tissue block with a size of 10 * 10 * 5mm.
3) For cells, the number of cells must be ≥106