detailed informationParaffin embedding steps 1 tissue dehydration, penetration and embedding 1 dehydration: 70% ethanol (60min) → 80% ethanol (60min) → 90% ethanol (60min) → 95% ethanol (30min) → 95% ethanol (30min) → 100% ethanol (30min) → 100% ethanol for 30min. 2 Transparent (dealcoholized): 1/2 ethanol + 1/2 xylene (generally 2000ul each, the same below) (30 min) → xylene (15min) → xylene (10-15min). 3 Penetration (impregnation): 1/2 xylene + 1/2 paraffin (60min, 55 ° C) → Paraffin I (60min, 55 ° C) () 4 Embedding: The tissue is placed in a paraffin-containing mold, placed in position, and cooled on the cold stage of the paraffin embedder. 5. 2 Slices and patches 1. Slicing: Use a microtome. Commonly used slicers are rotary and flat push. It is easy to cut out continuous slices with a rotary type. The slice thickness is adjusted according to the purpose of filming. The thickness of teaching specimens is mostly 5 ~ 6μm. 2. Patch drying: After paraffin sections are flattened in warm water, they are moved on glass slides, numbered, and baked at 65 ° C for 1 h, and then ready for dyeing. After staining, it is sealed with gum and cover sheet, which can be stored permanently. Note: The above content is the routine process of the automatic embedding machine, and it can be adjusted by itself in case of special organizations.
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