In vivo optical imaging technology can be used to carry out in vivo functional research directly at the level of live animals, observe the expression or interaction of specific cells, genes and molecules in real time, detect multiple molecular events at the same time, and use labeled transgenic animal models to study the occurrence and development of diseases Process, etc. The combination of in vivo optical imaging technology and transgenic animals can trace many important cells and molecules in real time, especially tumor cells, immune-related cells and mediators, so as to understand their role and provide clues to reveal the pathological processes of various diseases. The ability of non-invasive detection of reporter gene expression by in vivo optical imaging technology has huge advantages compared with traditional methods of tissue staining and enzyme activity analysis after experimental animals are sacrificed, because experimental animals do not need to Time points were slaughtered in batches. In vivo optical imaging technology can obtain the overall data of all time points on the same experimental animal in the experiment, and can obtain more comprehensive data quickly with very few experimental animals. This greatly saves experimental animals, experimental time and experimental expenses. This has important application value for animal experiments. Because the same animal can be continuously tested at different time points, this minimizes the impact on experimental results due to individual differences between different experimental animals and errors in traditional detection methods. In vivo optical imaging technology can quickly detect multiple experimental animals at the same time, and the imaging time is less than 5 minutes, so that a large number of detection work can be performed with limited technical power. In addition, the sensitivity of in vivo optical imaging technology is extremely high. The sensitivity of in vivo optical imaging technology to detect tumor cells in vivo even exceeds that of flow cytometry in vitro. Combining these characteristics, the purchase of small animal living optical imaging systems will be maximized and utilized at the highest level.