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QPCR extraction-free detection kit

source:未知 author:admin Release time:2020-02-15 01:19 Browse volume:
Direct extraction of DNA without qPCR (probe method) is environmentally friendly!
 
SHFB
qPCR - EASY Kit
First in China and International Technology Leader
 
Direct qPCR (probe method) kit (1 step method)
Article number: SHFB-UQ088
product description
This kit is designed for use in hands-free DNA for qPCR (probe) amplification of human (or animal) tissues, body fluids, or cultured cells. The kit includes all reagents needed for rapid preparation of sample genomic DNA and qPCR (probe method). The method of this kit is simple and fast. It only needs "1 step method" to obtain extraction-free DNA. The entire process does not require extraction with organic solvents and precipitation with anhydrous ethanol. Simple, fast, and repeatable.
The 2 × qPCR-Easy Master Mix provided by this kit can efficiently amplify samples and is a qPCR reagent with strong amplification compatibility. Contains Taq plus DNA polymerase, dNTPs, Mg2 +, reaction buffer, qPCR reaction enhancer and optimizer. Users only need to prepare primers and probes to perform qPCR reactions. It is simple and fast, high sensitivity, strong specificity, good stability, etc., suitable for high throughput and standardization.
Features
² Simple and fast operation: one step operation can get the DNA of the sample to be tested, and directly perform qPCR reaction
² Safe and non-carcinogens: no phenol-chloroform and other carcinogenic and toxic compounds, safe and non-toxic
² Green products: no consumables such as spin columns, less plastic waste, green
² Good repeatability to avoid cross-contamination: Simplified process and avoid cross-contamination between samples
² Suitable for fresh / frozen tissue, paraffin sections, blood, body fluids and cells
² Recommended simple and stable sampling kit: with SHFB tissue sampling kit / SHFB liquid sampling kit, it can stably store and transport samples at room temperature
² If you use other unlisted samples, please contact technical support (Tel: 18317162905)
Example: Using this SHFB qPCR-EASY Kit, the amplification curve of direct qPCR probe method for extraction-free DNA (11 biological samples are shown, all meet the requirements. The value of Cq / Ct is related to the cell density of the sample): (Jointly developed Unit: Shanghai Honghui Bio)

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